AF9 antibody (AA 1-150)

Catalog No. ABIN6143888

This Rabbit Polyclonal antibody specifically detects AF9 in WB. It exhibits reactivity toward Human.

Quick Overview for AF9 antibody (AA 1-150) (ABIN6143888)

Target: AF9 (MLLT3) (Protein AF-9 (MLLT3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AF9 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AF9 Antibody

Binding Specificity: AA 1-150

Sequence: MASSCAVQVK LELGHRAQVR KKPTVEGFTH DWMVFVRGPE HSNIQHFVEK VVFHLHESFP RPKRVCKDPP YKVEESGYAG FILPIEVYFK NKEEPRKVRF DYDLFLHLEG HPPVNHLRCE KLTFNNPTED FRRKLLKAGG DPNRSIHTSS

Cross-Reactivity: Human

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human MLLT3/AF9 (NP_004520.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000

Comment:

HIGH QUALITY

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for AF9

Target: AF9 (MLLT3) (Protein AF-9 (MLLT3))

Alternative Name: MLLT3

Background: Chromatin reader component of the super elongation complex (SEC, a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr, and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr. Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively, but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones. Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 'Lys-79' methylation (H3K79me. Plays a key role in hematopoietic stem cell (HSC maintenance by preserving, rather than confering, HSC stemness. Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L.,MLLT3,AF9,YEATS3,Epigenetics & Nuclear Signaling,Cancer,MLLT3

Molecular Weight: 63 kDa

Gene ID: 4300

UniProt: P42568